Abstract: Using observational data from the Parkes 64 m radio telescope at 1369 MHz, the single pulse data of the PSR J0934-5249 has been conducted in detail. The observational results show that this pulsar has both Pulse nulling and subpulse Drifting phenomena. Initially, the pulsar's pulse nulling phenomenon is investigated, successfully separating null pulses and burst pulses, and the NF (Nulling Fraction) is calculated to be 6.3%\pm0.4%, and the durations of both null and burst pulses are obtained. The results show that the longest duration of burst pulses is more than 150 pulse periods, while the duration of null pulses is no more than 4 pulse periods. In addition, this pulsar shows a quasi-periodic nulling phenomenon with a periodic value of approximately 33 pulse periods. Furthermore, analysis using longitude-resolved fluctuation spectra and two-dimensional fluctuation spectra reveals the presence of a clear subpulse drifting phenomenon in PSR J0934-5249. The vertical separation of the drift bands is calculated as P_3=3.85P\pm0.03P (P is the rotation period of this pulsar). The horizontal separation of the drift bands is P_2=5.2^\circ\pm1.0^\circ. Finally, polarization information for PSR J0934-5249 at a frequency of 1369 MHz is given. It is found that the pulsar's polarization position angle is consistent with the rotating vector model, and the observational geometry of the pulsar is well constrained based on kinematic effects.